1CRU
SOLUBLE QUINOPROTEIN GLUCOSE DEHYDROGENASE FROM ACINETOBACTER CALCOACETICUS IN COMPLEX WITH PQQ AND METHYLHYDRAZINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-04-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.579, 92.658, 85.727 |
| Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.19500 |
| RMSD bond length | 0.027 * |
| RMSD bond angle | 2.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.034 | 0.340 |
| Total number of observations | 580379 * | |
| Number of reflections | 142076 | |
| <I/σ(I)> | 30.2 | |
| Completeness [%] | 97.8 | 91.2 |
| Redundancy | 4.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9.2 | 293 | Oubrie, A., (1999) J.Mol.Biol., 289, 319. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | PQQ | 0.1 (mM) | |
| 3 | 1 | drop | PEG6000 | 8 (%(w/v)) | |
| 4 | 1 | drop | Tris-glycine | 50 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 20-23 (%(w/v)) | |
| 6 | 1 | reservoir | 120 (mM) | ||
| 7 | 1 | reservoir | 3 (mM) | ||
| 8 | 1 | reservoir | Tris-glycine | 50 (mM) |
