1CR5
N-TERMINAL DOMAIN OF SEC18P
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-06-09 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.710, 32.140, 94.850 |
| Unit cell angles | 90.00, 118.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.23 * |
| Rwork | 0.230 |
| R-free | 0.27700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.344 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.040 | 0.115 |
| Total number of observations | 176811 * | |
| Number of reflections | 22366 | |
| <I/σ(I)> | 23.9 | |
| Completeness [%] | 98.8 | 99.8 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 277 | PEG 8000, sodium phosphate, dimethyl sulfoxide, ATP, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 10 (mM) | |
| 2 | 1 | drop | protein | 10 (mg/ml) | |
| 3 | 1 | reservoir | sodium phosphate | 50 (mM) | |
| 4 | 1 | reservoir | DMSO | 5-10 (%) | |
| 5 | 1 | reservoir | PEG8000 | 5 (%) | |
| 6 | 1 | reservoir | ATP | 5 (mM) |
