1CR0

CRYSTAL STRUCTURE OF THE HELICASE DOMAIN OF THE GENE4 PROTEIN OF BACTERIOPHAGE T7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	113
Detector technology	CCD
Collection date	1999-01-08
Detector	ADSC
Spacegroup name	P 61
Unit cell lengths	80.600, 80.600, 85.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.254
Rwork	0.247
R-free	0.30200 *
RMSD bond length	0.013
RMSD bond angle	1.900
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.399
Total number of observations	144142 *
Number of reflections	13523
<I/σ(I)>	26
Completeness [%]	97.8	99.9
Redundancy	10.6	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	298	AMMONIUM SULFATE, ACES, pH 9.00, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	298	AMMONIUM SULFATE, ACES, pH 9.00, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-35 (mg/ml)
2	1	drop	lithium sulfate	10 (mM)
3	1	drop	Tris-HCl	20 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	reservoir	ammonium sulfate	1.4-1.6 (M)
6	1	reservoir	ACES	100 (mM)