1CQX
Crystal structure of the flavohemoglobin from Alcaligenes eutrophus at 1.75 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1993-12-01 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.200, 85.800, 103.900 |
Unit cell angles | 90.00, 81.80, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.750 |
Rwork | 0.182 |
R-free | 0.21500 |
RMSD bond length | 0.007 |
RMSD bond angle | 22.600 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | MLPHARE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.052 | 0.151 |
Number of reflections | 196672 | |
<I/σ(I)> | 8.1 | |
Completeness [%] | 90.5 | 62.7 |
Redundancy | 2.4 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 281 | protein solution: TRIS, potassium chloride, DTT, plus precipitant; precipitant: PEG 3350, sodium chloride, sodium citrate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 0.02 (M) | |
3 | 1 | drop | 0.01 (M) | ||
4 | 1 | drop | dithiothreitol | 2 (mM) | |
5 | 1 | reservoir | PEG3350 | 14 (%) | |
6 | 1 | reservoir | 0.2 (M) | ||
7 | 1 | reservoir | sodium citrate | 0.05 (M) |