1CQ3
STRUCTURE OF A SOLUBLE SECRETED CHEMOKINE INHIBITOR, VCCI, FROM COWPOX VIRUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-05-25 |
| Detector | OTHER |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.699, 64.423, 245.288 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.24200 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.071 | 0.157 |
| Number of reflections | 40519 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 86.7 | 52.8 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 12 * | 22% (w/v) PEG8K,10% Glycerol, 50 mM Tris-HCl pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 10 (mM) | |
| 2 | 1 | drop | protein | 4 (mg/ml) | |
| 3 | 1 | reservoir | PEG8000 | 20-22 (%(v/v)) | |
| 4 | 1 | reservoir | glycerol | 10 (%(w/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 25 (mM) |
