1CPO
CHLOROPEROXIDASE
Experimental procedure
| Collection date | 1994-04-28 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.774, 71.363, 91.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.22400 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.557 |
| Data reduction software | XENGEN |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.082 * |
| Total number of observations | 152222 * |
| Number of reflections | 29366 |
| Completeness [%] | 94.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | Sundaramoorthy, M., (1995) Acta Crystallogr.,Sect.D, 51, 842. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 20 (%) | |
| 2 | 1 | reservoir | 0.31 (M) | can be replaced by LiCl or KCl | |
| 3 | 1 | reservoir | potassium phosphate | 0.01 (M) | |
| 4 | 1 | drop | PEG6000 | 10 (%) | |
| 5 | 1 | drop | 0.155 (M) | can be replaced by LiCl or KCl | |
| 6 | 1 | drop | potassium phosphate | 0.005 (M) | |
| 7 | 1 | drop | protein | 5 (mg/ml) | |
| 8 | 1 | drop | phosphate | 0.005 (M) |
