1CPN

NATIVE-LIKE IN VIVO FOLDING OF A CIRCULARLY PERMUTED JELLYROLL PROTEIN SHOWN BY CRYSTAL STRUCTURE ANALYSIS

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	65.470, 41.560, 39.570
Unit cell angles	90.00, 111.46, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.164
Rwork	0.164
RMSD bond length	0.015
RMSD bond angle	2.769
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.047 *
Number of reflections	16674 *
Completeness [%]	90.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	CpMAC-57	5 (mg/ml)
2	1	1	sodium acetate	10 (mM)
3	1	1	EDTA	2 (mM)
4	1	1		5 (mM)
5	1	2	PEG8000	20 (%)
6	1	2	isopropyl alcohol	5 (%(v/v))
7	1	2	Tris-HCl	0.1 (M)