1CP7
AMINOPEPTIDASE FROM STREPTOMYCES GRISEUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X26C |
| Synchrotron site | NSLS |
| Beamline | X26C |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10-26 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 61.750, 61.750, 146.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 1.580 |
| R-factor | 0.144 |
| Rwork | 0.144 |
| R-free | 0.17100 |
| Structure solution method | OTHER |
| Starting model (for MR) | PDB ENTERY 1XJO |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.045 | 0.141 |
| Number of reflections | 37375 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 94.0 | 63.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | Greenblatt, H.M., (1997) J.Mol.Biol., 265, 620. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 16 (%(w/v)) | |
| 4 | 1 | drop | Tris | 10 (mM) | |
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | drop | 1 (mM) |
