1CP6
1-BUTANEBORONIC ACID BINDING TO AEROMONAS PROTEOLYTICA AMINOPEPTIDASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-15 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 109.200, 109.200, 97.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.22900 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1amp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.820 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | X-PLOR (4.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.134 | 0.378 |
| Total number of observations | 163530 * | |
| Number of reflections | 23658 | |
| Completeness [%] | 84.2 | 46.7 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 100 MM TRIS PH 8.0, 100 MM KSCN, 4.5 M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | KSCN | 10 (mM) | |
| 4 | 1 | drop | 0.4 (M) | ||
| 5 | 1 | drop | BuBA | 4-fold molar excess | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | KSCN | 100 (mM) | |
| 8 | 1 | reservoir | 4.5 (M) |
