1COW
BOVINE MITOCHONDRIAL F1-ATPASE COMPLEXED WITH AUROVERTIN B
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-08 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 283.400, 107.600, 140.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-free | 0.28500 * |
| RMSD bond length | 0.015 |
| RMSD bond angle | 17.200 * |
| Data reduction software | MOSFLM |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 * |
| High resolution limit [Å] | 3.100 * |
| Rmerge | 0.075 |
| Number of reflections | 76643 |
| Completeness [%] | 97.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.2 * | 22-24 * | Lutter, R., (1993) J.Mol.Biol., 229, 787. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 2 | magnesium sulphate | 20 (mM) | |
| 10 | 1 | 1 | magnesium chloride | 8 (mM) | |
| 11 | 1 | 1 | AMP-PNP | 0.500 (mM) | * |
| 12 | 1 | 1 | ADP | 0.010 (mM) | * |
| 13 | 1 | 2 | * solutions | half of concentration * solutions | |
| 14 | 1 | 2 | PEG6000 | 9 (%(w/v)) | |
| 15 | 1 | 2 | 2-mercaptoethanol | 5 (mM) | |
| 2 | 1 | 1 | deuterium oxide | 99.9 (%) | |
| 3 | 1 | 1 | Tris-HCl | 100 (mM) | * |
| 4 | 1 | 1 | PEG6000 | 14 (%(w/v)) | |
| 5 | 1 | 1 | sodium chloride | 400 (mM) | * |
| 6 | 1 | 1 | EDTA | 2 (mM) | * |
| 7 | 1 | 1 | sodium azide | 0.04 (%(w/v)) | * |
| 8 | 1 | 1 | PMSF | 0.02 (%(w/v)) | * |
| 9 | 1 | 1 | dithiothreitol | 10 (mM) | * |
