1COB

CRYSTAL STRUCTURE SOLUTION AND REFINEMENT OF THE SEMISYNTHETIC COBALT SUBSTITUTED BOVINE ERYTHROCYTE ENZYME SUPEROXIDE DISMUTASE AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	50.990, 147.630, 47.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.176
RMSD bond length	0.010 *
RMSD bond angle	1.748 *
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.068 *
Total number of observations	18964 *
Number of reflections	116401 *
Completeness [%]	75.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Cu,Co b-SOD	16 (mg/ml)
2	1	drop	glycylglycine	0.050 (M)
3	1	drop	PEG4000	8 (%(w/v))
4	1	drop		0.1 (M)
5	1	reservoir	PEG	16 (%(w/v))