1CNU

PHOSPHORYLATED ACTOPHORIN FROM ACANTAMOEBA POLYPHAGA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-09-01
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	40.200, 46.500, 68.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.250
R-factor	0.204 *
Rwork	0.204
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ACTOPHORIN
RMSD bond length	0.012
RMSD bond angle	0.037
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	EPMR
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.225
Rmerge	0.091
Number of reflections	6441
Completeness [%]	96.2
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir	PEG8000	20-25 (%)