Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 1997-12-01 |
| Detector | SIEMENS |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 52.340, 82.820, 166.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.270 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fbe |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.160 * |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.400 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.055 | |
| Number of reflections | 15519 | |
| Completeness [%] | 90.0 | 56 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 37 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-malonate | 2.5 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | F6P | 5 (mM) | |
| 6 | 1 | reservoir | Tris-malonate | 2.5 (mM) | |
| 7 | 1 | reservoir | PEG3350 | 6 (%(w/v)) |
