1CN3
INTERACTION OF POLYOMAVIRUS INTERNAL PROTEIN VP2 WITH MAJOR CAPSID PROTEIN VP1 AND IMPLICATIONS FOR PARTICIPATION OF VP2 IN VIRAL ENTRY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 300 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 219.000, 219.000, 99.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.230 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.090 |
| Number of reflections | 25000 |
| Completeness [%] | 95.0 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.4 * | pH 4.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 15 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.5 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | glycerol | 10 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
