1CM7

3-ISOPROPYLMALATE DEHYDROGENASE FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	55.800, 86.900, 83.700
Unit cell angles	90.00, 93.30, 90.00

Refinement procedure

Resolution	6.000 - 2.060
Rwork	0.173
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1 IPD
RMSD bond length	0.010
RMSD bond angle	1.700
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.060
Rmerge	0.058 *
Total number of observations	89369 *
Number of reflections	36432
Completeness [%]	79.0 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		15 MG/ML PROTEIN IN 15% PEG 4K, 50 MM TRIS PH = 7.5, 0.35 M MGCL2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	PEG4000	15 (%(w/v))
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		0.35 (M)