1CLL
CALMODULIN STRUCTURE REFINED AT 1.7 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | P 1 |
| Unit cell lengths | 30.170, 53.600, 25.140 |
| Unit cell angles | 93.62, 97.30, 89.17 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.225 * |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.032 |
| Phasing software | MERLOT |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 * |
| Rmerge | 0.329 * |
| Number of reflections | 15417 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | ||
| 10 | 1 | reservoir | ethanol | 15 (%) | |
| 11 | 1 | reservoir | NaOAc | 50 (mM) | |
| 12 | 1 | reservoir | 50 (mM) | ||
| 13 | 1 | reservoir | 5 (mM) | ||
| 2 | 1 | drop | calmodulin | ||
| 3 | 1 | drop | protein | 12 (mg/ml) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | NaOAc | 50 (mM) | |
| 7 | 1 | drop | MPD | 17.5 (%(v/v)) | |
| 8 | 1 | drop | ethanol | 7.5 (%(v/v)) | |
| 9 | 1 | reservoir | MPD | 35 (%) |
