1CL5
CRYSTAL STRUCTURE OF PHOSPHOLIPASE A2 FROM DABOIA RUSSELLI PULCHELLA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 1998-09-01 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 77.048, 92.347, 77.049 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
Rwork | 0.188 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pp2 |
RMSD bond length | 0.007 |
RMSD bond angle | 26.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.400 * | 2.400 * |
Rmerge | 0.098 | 0.116 * |
Number of reflections | 10169 * | |
<I/σ(I)> | 16.02 | 14.2 |
Completeness [%] | 98.0 * | 98.4 |
Redundancy | 3.9 * | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7 | 298 * | PROTEIN WAS CRYSTALLISED FROM 1.2 AMMONIUM SULPHATE BUFFER WITH 20MM SODIUM CACODYLATE, 2MM CALCIUM CHLORIDE AND 3% DIOXANE, pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protease | 15 (mg/ml) | |
2 | 1 | drop | sodium cacodylate | 20 (mM) | |
3 | 1 | drop | calcium chloride | 1 (mM) | |
4 | 1 | drop | dioxane | 3 (%(v/v)) | |
5 | 1 | reservoir | ammonium sulfate | 1.2 (M) |