1CKQ
PRE-TRANSITION STATE ECO RI ENDONUCLEASE/COGNATE DNA (TCGCGAATTCGCG) COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-04-15 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 118.110, 118.110, 49.450 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.28600 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1eri |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 1.910 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.124 | 0.446 |
| Number of reflections | 32270 | |
| <I/σ(I)> | 22.2 | 2 |
| Completeness [%] | 99.1 | 91.1 |
| Redundancy | 10.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
