1CKB

STRUCTURAL BASIS FOR THE SPECIFIC INTERACTION OF LYSINE-CONTAINING PROLINE-RICH PEPTIDES WITH THE N-TERMINAL SH3 DOMAIN OF C-CRK

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-11-18
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 41
Unit cell lengths	47.400, 47.400, 29.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.183
Rwork	0.183
RMSD bond length	0.011
RMSD bond angle	1.900 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.900 *
Rmerge	0.094 *
Total number of observations	50442 *
Number of reflections	5289
Completeness [%]	95.5
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *	21 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	85 (mg/ml)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	ammonium acetate	0.2 (M)
4	1	drop	Sos peptide	9.0 (mM)
5	1	reservoir	PEG4000	28-32 (%)