1CJR

X-RAY CRYSTALLOGRAPHIC STUDIES OF DENATURATION IN RIBONUCLEASE S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Spacegroup name	P 31 2 1
Unit cell lengths	44.650, 44.650, 98.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.234 *
Rwork	0.245
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	24.700 *
Data reduction software	AUTOMAR
Data scaling software	XDS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.400
High resolution limit [Å]	2.200	2.200
Rmerge	0.070
Number of reflections	4968
Completeness [%]	86.0	86
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.5 *		Kim, E.E., (1992) Biochemistry, 31, 12304. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12.5 (mg/ml)
2	1	drop		6.0 (M)
3	1	drop	sodium acetate	0.1 (M)
4	1	reservoir		80 (%sat)
5	1	reservoir	sodium acetate	0.1 (M)