1CJM
HUMAN SULT1A3 WITH SULFATE BOUND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 56.400, 56.400, 191.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.276 * |
| Rwork | 0.276 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 * | 0.330 * |
| Total number of observations | 63776 * | |
| Number of reflections | 15080 | |
| <I/σ(I)> | 14.3 | 2.7 |
| Completeness [%] | 97.0 | 86.8 * |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 20 * | 0.5 M LITHIUM SULFATE AND 5-7% POLYETHYLENE GLYCOL 8000, pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium phosphate | 5 (mM) | |
| 2 | 1 | drop | dithiothreitol | 1 (mM) | |
| 3 | 1 | drop | protein | 15-20 (mg/ml) | |
| 4 | 1 | reservoir | lithium sulfate | 0.5 (M) | |
| 5 | 1 | reservoir | PEG8000 | 5-7 (%(w/v)) |
