1CJC
STRUCTURE OF ADRENODOXIN REDUCTASE OF MITOCHONDRIAL P450 SYSTEMS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.810, 62.530, 78.370 |
| Unit cell angles | 90.00, 106.76, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.700 |
| Rwork | 0.188 |
| R-free | 0.22300 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.024 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.054 | 0.295 |
| Number of reflections | 56738 | |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 93.0 | 76 |
| Redundancy | 4.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | DROPLET: 4 MG/ML PROTEIN, 8% PEG8000, 5% GLYCEROL, 100 MM CALCIUM ACETATE, 50 MM SODIUM-CACODYLATE PH 6.5. RESERVOIR: 12 % PEG8000, 100 MM CALCIUM ACETATE, 50 MM SODIUM CACODYLATE PH 6.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | (mg/ml) | |
| 2 | 1 | drop | sodium cacodylate | (mM) | |
| 3 | 1 | drop | calcium acetate | (mM) | |
| 4 | 1 | drop | PEG8000 | (%(w/v)) | |
| 5 | 1 | drop | glycerol | (%(v/v)) | |
| 6 | 1 | reservoir | PEG8000 | (%(w/v)) | |
| 7 | 1 | reservoir | sodium cacodylate | (mM) | |
| 8 | 1 | reservoir | calcium acetate | (mM) |
