1CIY
INSECTICIDAL TOXIN: STRUCTURE AND CHANNEL FORMATION
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.000, 111.500, 154.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.250 |
| R-factor | 0.168 * |
| Rwork | 0.163 |
| R-free | 0.24800 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.930 * |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 154.000 |
| High resolution limit [Å] | 2.250 |
| Rmerge | 0.059 |
| Total number of observations | 86937 * |
| Number of reflections | 33090 |
| Completeness [%] | 74.0 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 10 * | Borisova, S., (1994) J.Mol.Biol., 243, 530. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | CAPS | 20-30 (mM) | |
| 3 | 1 | drop | MPD | 3-4 (%) | |
| 4 | 1 | reservoir | MPD | 10 (%) | |
| 5 | 1 | reservoir | CAPS | 100 (mM) |
