1CIU

THERMOSTABLE CGTASE FROM THERMOANAEROBACTERIUM THERMOSULFURIGENES EM1 AT PH 8.0.

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1995-01-23
Detector	MACSCIENCE
Spacegroup name	P 21 21 21
Unit cell lengths	73.900, 97.400, 115.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.179
R-free	0.24900 *
RMSD bond length	0.004
RMSD bond angle	0.900
Data reduction software	XDS
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.300 *
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.061
Total number of observations	119291 *
Number of reflections	31189 *
Completeness [%]	82.4 *	66.6 *
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.4 (mg/ml)
2	1	drop	sodium acetate	10 (mM)
3	1	reservoir	ammonium sulfate	19-22 (%sat)