1CIQ

COMPLEX OF TWO FRAGMENTS OF CI2, RESIDUES 1-40 AND 41-64

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-11-14
Detector	MARRESEARCH
Spacegroup name	P 6 2 2
Unit cell lengths	69.170, 69.170, 53.910
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.200
R-factor	0.18
RMSD bond length	0.020
RMSD bond angle	0.040
Data reduction software	MOSFLM
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.960
High resolution limit [Å]	2.200
Rmerge	0.005 *	0.106 *
Total number of observations	23355 *
Number of reflections	4179
Completeness [%]	99.7	97.1 *
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *	23 *	drop solution is mixed 1:1 by volume with reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	reconstituted fragment complex	22 (mg/ml)
2	1	reservoir	ammonium sulfate	40 (%sat)
3	1	reservoir	Tris-HCl	50 (mM)