1CID

CRYSTAL STRUCTURE OF DOMAINS 3 & 4 OF RAT CD4 AND THEIR RELATIONSHIP TO THE NH2-TERMINAL DOMAINS

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	77.800, 77.800, 82.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.233 *
RMSD bond length	0.022
RMSD bond angle	0.081
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.065 *
Total number of observations	41516 *
Number of reflections	7274 *
Completeness [%]	99.1 *
Redundancy	5.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	ammonium sulfate	58-62 (%sat)
4	1	reservoir	Tris-HCl	20 (mM)