1CHN

MAGNESIUM BINDING TO THE BACTERIAL CHEMOTAXIS PROTEIN CHEY RESULTS IN LARGE CONFORMATIONAL CHANGES INVOLVING ITS FUNCTIONAL SURFACE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	29.900, 44.400, 46.900
Unit cell angles	90.00, 102.90, 90.00

Refinement procedure

Resolution	10.000 - 1.760
R-factor	0.191
Rwork	0.191
RMSD bond length	0.019
RMSD bond angle	1.720
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.760 *
Rmerge	0.070 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	magnesium acetate	0.2 (M)
2	1	reservoir	PEG6000	20 (%)
3	1	reservoir	sodium cacodylate	0.1 (M)