1CGX

SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYXOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	120.327, 110.847, 66.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 * - 2.590 *
R-factor	0.152 *
RMSD bond length	0.011 *
RMSD bond angle	2.180 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000 *
High resolution limit [Å]	2.590 *	2.590 *
Rmerge	0.071 *
Total number of observations	84866 *
Number of reflections	26761 *
Completeness [%]	95.1 *	78.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *