1CGW

SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYCOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	121.091, 111.180, 66.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.000 * - 2.530 *
R-factor	0.16
RMSD bond length	0.011 *
RMSD bond angle	2.250 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000 *
High resolution limit [Å]	2.530 *	2.530 *
Rmerge	0.078 *
Total number of observations	95261 *
Number of reflections	27835 *
Completeness [%]	90.3 *	30.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *