1CGT

STRUCTURE OF CYCLODEXTRIN GLYCOSYLTRANSFERASE REFINED AT 2.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	94.800, 104.700, 114.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.166
Rwork	0.166
RMSD bond length	0.014
RMSD bond angle	2.780
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.119 *
Total number of observations	387934 *
Number of reflections	70862 *
Completeness [%]	96.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	50 (%(v/v))
2	1	drop	CGTase	7-15 (mg/ml)
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	PEG1500	0.5 (%)
5	1	reservoir	ammonium sulphate	0.9-1.0 (M)
6	1	reservoir	CGTase	7-15 (mg/ml)
7	1	reservoir	Tris-HCl	50 (mM)
8	1	reservoir	PEG1500	0.5 (%)