1CGP
CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Detector technology | AREA DETECTOR |
| Detector | SDMS |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 138.000, 152.600, 76.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| Rwork | 0.235 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.400 |
| Refinement software | X-PLOR |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 5 | pH 5.00, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 1 | DTT | ||
| 11 | 1 | 2 | WATER | ||
| 12 | 1 | 2 | PEG 3350 | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | CACL2 | ||
| 5 | 1 | 1 | MES | ||
| 6 | 1 | 1 | CAMP | ||
| 7 | 1 | 1 | SPERMINE | ||
| 8 | 1 | 1 | N-OCTYL-BETA-D-GLUCOPYRANOSIDE | ||
| 9 | 1 | 1 | NA AZIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | PEG | 5 (%(w/v)) | ||
| 4 | 1 | cAMP | 2 (mM) |
