1CGC

DOUBLE HELIX CONFORMATION GROOVE DIMENSIONS AND LIGAND BINDING POTENTIAL OF A G/C-STRETCH IN B-DNA

Experimental procedure

Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Detector technology	DIFFRACTOMETER
Detector	ENRAF-NONIUS CAD4
Spacegroup name	H 3
Unit cell lengths	54.070, 54.070, 44.590
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.167
Refinement software	NUCLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.200	2.200 *
Rmerge	0.075 *
Number of reflections	2086
Completeness [%]	78.0 *	68 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	7	4 *	pH 7.00, MICRODIALYSIS, temperature 277.00K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	WATER
2	1	1	BIS-TRIS-PROPANE_HCL
3	1	1	MGCL2
4	1	2	WATER
5	1	2	MPD

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1		DNA	2 (mM)
2	1		Tris-HCl	10 (mM)
4	1		Tris-HCl	10 (mM)
6	1	2	MPD	24 (%(v/v))