1CG9

COMPLEX RECOGNITION OF THE SUPERTYPIC BW6-DETERMINANT ON HLA-B AND-C MOLECULES BY THE MONOCLONAL ANTIBODY SFR8-B6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-07-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	45.550, 181.530, 141.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.700
Rwork	0.201
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a9e
RMSD bond length	0.004
RMSD bond angle	1.130
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	16292
<I/σ(I)>	10.6	2.3
Completeness [%]	98.4	99.6
Redundancy	2.75

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.9		4 M SODIUM FORMATE, PH 7.9