1CG8

CO Form Hemoglobin from Dasyatis Akajei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	298
Collection date	1998-06-01
Spacegroup name	C 2 2 21
Unit cell lengths	57.660, 100.070, 107.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.197 *
Rwork	0.197
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hhb
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.049	0.210
Total number of observations	169395 *
Number of reflections	23946	2163 *
Completeness [%]	95.9	86.8
Redundancy	7	3.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2.1-2.3 (M)
2	1	1	haemoglobin	2 (%)