1CFT
ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-UNRELATED D-PEPTIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1995-10-01 |
Detector | MARRESEARCH |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 106.060, 106.060, 294.130 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 65.000 - 2.800 |
R-factor | 0.237 * |
Rwork | 0.237 |
R-free | 0.32800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 0.045 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | CCP4 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 91.000 | 2.900 |
High resolution limit [Å] | 2.600 | 2.800 |
Rmerge | 0.104 | 0.310 |
Number of reflections | 30960 | |
<I/σ(I)> | 5.6 | 1.4 |
Completeness [%] | 97.0 | 98 |
Redundancy | 4.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | 1.8 M (NH4)2 SO4, 0.1 M 3-(N- MORPHOLINO)-PROPANESULFONIC AC, pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Fab | 10 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
3 | 1 | reservoir | 2-(N-morpholino)-propanesulfonic acid | 0.1 (M) | |
4 | 1 | reservoir | 0.02 (%) |