1CFQ
ANTI-P24 (HIV-1) FAB FRAGMENT CB41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.340, 55.600, 75.670 |
| Unit cell angles | 90.00, 118.91, 90.00 |
Refinement procedure
| Resolution | 65.000 - 2.800 |
| R-factor | 0.221 * |
| Rwork | 0.221 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.046 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.560 |
| Rmerge | 0.154 | |
| Number of reflections | 18017 | |
| <I/σ(I)> | 1.36 | |
| Completeness [%] | 84.0 | 89 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 12% POLYETHYLENE MONOMETHYL ETHER 2000, 5 MM NICL2, 0.1 M TRIS-HCL PH 8.5, 0.02% NAN3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab | 10 (mg/ml) | |
| 2 | 1 | reservoir | mPEG2000 | 12 (%) | |
| 3 | 1 | reservoir | 5 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 5 | 1 | reservoir | 0.02 (%) |
