1CFN

ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-RELATED PEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	288
Detector technology	IMAGE PLATE
Collection date	1995-10-01
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	105.440, 105.440, 295.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	90.000 - 2.650
R-factor	0.277 *
Rwork	0.277
R-free	0.32500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2HFL
RMSD bond length	0.011
RMSD bond angle	0.037
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	CCP4

Data quality characteristics

	Overall
Low resolution limit [Å]	91.000
High resolution limit [Å]	2.600
Rmerge	0.107 *
Number of reflections	29821
Completeness [%]	98.0 *
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		1.8 M (NH4)2 SO4, 0.1 M 3-(N- MORPHOLINO)-PROPANESULFONIC AC, pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Fab	10 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8 (M)
3	1	reservoir	2-(N-morpholino)-propanesulfonic acid	0.1 (M)
4	1	reservoir		0.02 (%)