1CFB

CRYSTAL STRUCTURE OF TANDEM TYPE III FIBRONECTIN DOMAINS FROM DROSOPHILA NEUROGLIAN AT 2.0 ANGSTROMS

Experimental procedure

Spacegroup name	F 4 3 2
Unit cell lengths	241.800, 241.800, 241.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.000
R-factor	0.195 *
Rwork	0.202
R-free	0.23500 *
RMSD bond length	0.015
RMSD bond angle	1.900
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.050 *
Total number of observations	355936 *
Number of reflections	45766 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.6 (M)
2	1	reservoir		150 (mM)
3	1	reservoir	HEPES	100 (mM)