1CF7
STRUCTURAL BASIS OF DNA RECOGNITION BY THE HETERODIMERIC CELL CYCLE TRANSCRIPTION FACTOR E2F-DP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 127 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.300, 101.300, 73.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| R-factor | 0.229 * |
| Rwork | 0.223 |
| R-free | 0.28300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.750 |
| Refinement software | CNS (0.3A) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.054 * |
| Total number of observations | 41452 * |
| Number of reflections | 12901 |
| Completeness [%] | 96.5 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 4 * | 14-16% PEG 400, 25MM NACL, 50MM MES, 10MM DTT, PH=6.0, 4 DEGREES C, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 27-32 (%) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 20 (mM) | pH6.0 |
| 5 | 1 | drop | ternary complex | 0.3-0.6 (mM) | |
| 6 | 1 | drop | BTP | 20 (mM) | |
| 7 | 1 | drop | 100 (mM) | ||
| 8 | 1 | drop | dithiothreitol | 5 (mM) | |
| 9 | 1 | drop | ammonium acetate | 300 (mM) |
