1CF1

ARRESTIN FROM BOVINE ROD OUTER SEGMENTS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC
Spacegroup name	C 2 2 21
Unit cell lengths	168.850, 193.190, 191.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 2.800
R-factor	0.233
Rwork	0.233
R-free	0.24400
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.860
High resolution limit [Å]	2.800	2.800
Number of reflections	242164
<I/σ(I)>	11	2
Completeness [%]	84.7	42
Redundancy	3.8	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)
2	1	reservoir	ammonium acetate	200 (mM)
3	1	reservoir	isopropanol	22-25 (%)
4	1	drop	protein	10 (mg/ml)