1CEX

STRUCTURE OF CUTINASE

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	35.120, 67.360, 37.050
Unit cell angles	90.00, 93.90, 90.00

Refinement procedure

Resolution	15.000 - 1.000
R-free	0.11900
RMSD bond length	0.023
RMSD bond angle	0.028
Data reduction software	DENZO
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.000 *	1.000 *
Rmerge	0.039	0.598 *
Total number of observations	179064 *
Number of reflections	86474
Completeness [%]	93.3	99 *
Redundancy	2.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	20 *	Abergel, C., (1990) J. Mol. Biol., 215, 215. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	HEPES	0.1 (M)
3	1	reservoir	PEG1000	15-20 (%(w/v))