1CER
DETERMINANTS OF ENZYME THERMOSTABILITY OBSERVED IN THE MOLECULAR STRUCTURE OF THERMUS AQUATICUS D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AT 2.5 ANGSTROMS RESOLUTION
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1993-05-15 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 144.770, 148.770, 149.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 12.000 - 2.500 |
R-factor | 0.205 |
Rwork | 0.205 |
RMSD bond length | 0.021 |
RMSD bond angle | 26.320 * |
Data reduction software | R-AXIS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 2.500 * | 2.500 * |
Rmerge | 0.057 | |
Total number of observations | 267328 * | |
Number of reflections | 81628 | |
Completeness [%] | 72.0 | 30 * |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 8.4 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 10 (mg/ml) | |
10 | 1 | reservoir | isopropanol | 15 (%) | |
11 | 1 | reservoir | PEG3000 | 20-24 (%) | |
12 | 1 | reservoir | HEPES | 0.1 (M) | |
2 | 1 | drop | 50 (mM) | ||
3 | 1 | drop | beta-mercaptoethanol | 0.5 (mM) | |
4 | 1 | drop | EDTA | 0.5 (mM) | |
5 | 1 | drop | NAD | 1 (mM) | |
6 | 1 | drop | Tris-HCl | 1.25 (mM) | |
7 | 1 | drop | isopropanol | 7.5 (%) | |
8 | 1 | drop | PEG3000 | 10-12 (%) | |
9 | 1 | drop | HEPES | 0.05 (M) |