1CEO
CELLULASE (CELC) MUTANT WITH GLU 140 REPLACED BY GLN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.400, 84.330, 87.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.22200 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.086 |
| Total number of observations | 195009 * |
| Number of reflections | 34277 |
| Completeness [%] | 97.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG2000 | 22-25 (%) | |
| 2 | 1 | 1 | Tris-HCl | 0.1 (M) | |
| 3 | 1 | 1 | protein | (mg/ml) |
