1CEM

ENDOGLUCANASE A (CELA) CATALYTIC CORE, RESIDUES 33-395

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	50.118, 63.518, 104.974
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.650
R-factor	0.162
Rwork	0.162
R-free	0.19100
RMSD bond length	0.010
RMSD bond angle	1.400 *
Data reduction software	DENZO
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.650
Rmerge	0.050	0.155 *
Total number of observations	249508 *
Number of reflections	39868 *
Completeness [%]	96.9	94 *
Redundancy	6.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5 *		macroseeding, Souchon, H., (1996) Proteins: Struct.,Funct., Genet., 25, 134. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris	0.1 (M)	pH7.5
2	1	1		0.2 (M)
3	1	1	PEG4000	17 (%)
4	1	2	Tris-HCl	0.1 (M)	pH7.2
5	1	2	PEG8000	15 (%)