1CEG

CEPHALOTHIN COMPLEXED WITH DD-PEPTIDASE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1989-03-04
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	51.100, 67.300, 101.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.166
Rwork	0.166
R-free	0.21200
RMSD bond length	0.010
RMSD bond angle	1.900 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	1.760 *
Rmerge	0.051
Total number of observations	49740 *
Number of reflections	28901
Completeness [%]	72.0
Redundancy	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	drop	PEG8000	15 (%(w/v))
3	1	drop	phosphate	50 (mM)
4	1	reservoir	PEG8000	20 (%(w/v))