1CE0

TRIMERIZATION SPECIFICITY IN HIV-1 GP41: ANALYSIS WITH A GCN4 LEUCINE ZIPPER MODEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-10-15
Detector	MARRESEARCH
Spacegroup name	P 61
Unit cell lengths	50.785, 50.785, 119.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.221 *
Rwork	0.221
R-free	0.29400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gcm
RMSD bond length	0.006
RMSD bond angle	21.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.039	0.097
Total number of observations	22836 *
Number of reflections	6765
<I/σ(I)>	20.4	10.5
Completeness [%]	98.8	99.8
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	HEPES	0.1 (M)
3	1	drop		1.5 (M)
4	1	drop	glycerol	20 (%(v/v))
5	1	reservoir	glycerol	20 (%(v/v))