1CDI

STRUCTURES OF AN HIV AND MHC BINDING FRAGMENT FROM HUMAN CD4 AS REFINED IN TWO CRYSTAL LATTICES

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	133.550, 32.270, 46.070
Unit cell angles	90.00, 96.12, 90.00

Refinement procedure

Resolution	10.000 - 2.900
R-factor	0.183
Rwork	0.183
RMSD bond length	0.013
RMSD bond angle	0.038 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.900 *
Rmerge	0.089 *
Number of reflections	3999 *
Completeness [%]	91.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	20 *	referred to 'Ryu, S.-E.', (1990) Nature, 348, 419-426 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	20-25 (%)
2	1	drop	protein	20 (mg/ml)
3	1	drop	PEG3350	20-25 (%)