1CD8

CRYSTAL STRUCTURE OF A SOLUBLE FORM OF THE HUMAN T CELL CO-RECEPTOR CD8 AT 2.6 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 64 2 2
Unit cell lengths	101.000, 101.000, 55.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.192
Rwork	0.192
RMSD bond length	0.011
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.600 *	2.600 *
Rmerge	0.105 *	0.323 *
Total number of observations	19913 *
Number of reflections	4347 *	226 *
Completeness [%]		44 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	25-30 (%(w/v))
2	1	reservoir	ammonium sulphate	3.75 (%sat)
3	1	reservoir	sodium citrate	10 (mM)
4	1	drop	sCD8		0.001ml
5	1	drop	dH2O	5-10 (mg/ml)
6	1	drop	sodium citrate	10 (mM)