1CCZ

CRYSTAL STRUCTURE OF THE CD2-BINDING DOMAIN OF CD58 (LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN 3) AT 1.8-A RESOLUTION

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8983,0.946
Spacegroup name	P 32 2 1
Unit cell lengths	116.430, 116.430, 51.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.800
Rwork	0.202
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hng
RMSD bond length	0.011
RMSD bond angle	28.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.090	0.423
Total number of observations	187375 *
Number of reflections	37412 *
<I/σ(I)>	15.9	2.3
Completeness [%]	100.0	100
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	4.6		unpublished *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium chloride
2	1	1	sodium acetate	0.1 (M)