1CCD

REFINED STRUCTURE OF RAT CLARA CELL 17 KDA PROTEIN AT 3.0 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	52.070, 52.070, 109.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 3.000
R-factor	0.225
RMSD bond length	0.016
RMSD bond angle	0.062
Phasing software	X-PLOR
Refinement software	GPRLSA

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	3.000 *	3.000 *
Rmerge	2.000 *
Number of reflections	1733 *	381 *
Completeness [%]	85.0 *	63.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.1 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.1 (mg/ml)	0.040ml
2	1	drop	ammonium sulphate	60 (%(w/v)sat)	0.005ml
3	1	drop	Tris-HCl	10 (mM)
4	1	reservoir	ammonium sulfate	60 (%sat)
5	1	reservoir	Tris-HCl	10 (mg/ml)